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Role of poultry meat in a balanced diet aimed at maintaining health and wellbeing: an Italian consensus document

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However, if APAP overdose overwhelms the aforementioned detoxification pathways and depletes GSH to less than 30% of its normal level in the liver, NAPQI accumulates and forms covalent bonds with sulfhydryl groups on cysteine and lysine residues in the mitochondria of the hepatocytes [23,27]

Result: In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA