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Description

The slices were then blocked in 1 h with PBS containing 2.5% bovine serum albumin and 0.3% Triton X for 1 h at room temperature, and then incubated with 1:250 rabbit monoclonal anti-His-Tag and FluoTag-X2 anti-VGlut1 nanobodies directly conjugated to Atto 488 (#N1602

The results were expressed in micromoles per deciliter and reported as changes from the baseline values

4 .

Baron-Cohen also proposes an amygdala theory of autism and presents plausible abnormalities of the amygdala as a cause of ASD based on a review of MRI studies [43]