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Once anesthesia was induced using 5% isoflurane, cardiac blood collection was performed, followed by the rapid excision of prostate tissue, which was subsequently washed with pre-chilled 1.15% KCl solution

Elucidating the mechanisms underlying melatonin biosynthesis and its regulation in reproduction will provide valuable insights into fertility processes and enable the development of innovative treatments for reproductive disorders and infertility (14)

Of note, about 64% of the free drug was released within 4 h, while the maximum release from the NPs reached 89% in 12 h

PMID: 32029540, DOI: 10.1136/jnnp-2019-322534