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Practical strategies to circumvent the toxicity issues of UCNPs include the fabrication of ultra-small nanoparticles to increase biological elimination, surface functionalization with bio-biocompatible ligands or detoxifying reagents, and enhancement of the upconversion efficiency of UCNPs to decrease light dosage in practice

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1 Reactions are often executed in batch mode, where the reagents described above are combined and incubated until a limiting reagent is exhausted or inhibiting products accumulate

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