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Cells with laccase were enzymatically reacted with DAB in 0.1 M sodium cacodylate buffer at pH 7.4 for 320 min until the desired brown intensity color was achieved)

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Lastly, fungal laccases 19 , which have a broader substrate scope and higher redox potential than their bacterial counterparts, have been engineered for numerous industrial applications, including paper-pulp biobleaching, textile dye decolorization, bioremediation, food processing and biofuel cells 20