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Molecular mechanisms of curcumin renoprotection in experimental acute renal injury

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After a 1-day incubation, the cells were incubated for 1 h with 100 l of calcium indicator Fluo-4 from the FLIPR Calcium 5 Assay Kit (Molecular Devices) in Hanks BSS containing 20 mM HEPES-NaOH (pH7.5) and 2.5 mM probenecid